• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> PIP4K2 alpha Antibody / PIP4K2A

PIP4K2 alpha Antibody / PIP4K2A (F54749)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F54749-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54749-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
IHC testing of FFPE human brain tissue with PIP4K2 alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
›
IHC testing of FFPE human brain tissue with PIP4K2 alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PIP4K2 alpha antibody (green) and anti-Actin (red).
Western blot testing of human brain tissue lysate with PIP4K2 alpha antibody. Predicted molecular weight ~46 kDa.
Flow cytometry testing of human HeLa cells with PIP4K2 alpha antibody; Blue=isotype control, Green= PIP4K2 alpha antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P48426
Localization Cytoplasmic, nuclear
Applications Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Limitations This PIP4K2 alpha antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PIP4K2 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 329-363 from the human protein was used as the immunogen for the PIP4K2 alpha antibody.

Storage

Aliquot the PIP4K2 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all PIP4K2A antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.