- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
PILT Antibody / TJAP1 (RQ7550)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7550 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q5JTD0 |
| Localization | Cytoplasm (Golgi) |
| Applications | Western Blot : 0.5-1ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This PILT antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
PILT (Protein incorporated later into tight junctions), also called Tight junction-associated protein 1 and TJAP1, is a member of the membrane-associated guanylate kinase (MAGUK) family that contributes to the assembly and regulation of tight junctions. It is localized at epithelial and endothelial cell-cell junctions, where it helps maintain barrier integrity and supports polarity. A PILT antibody is frequently used to study cell adhesion, epithelial permeability, and intracellular signaling associated with tight junction complexes.
PILT contains characteristic protein interaction domains that enable binding with multiple partners, including claudins, ZO proteins, and signaling adaptors. These interactions allow PILT to bridge transmembrane receptors with cytosolic effectors, ensuring selective passage of molecules across epithelial barriers. Employing a PILT antibody provides researchers with a reliable tool for investigating barrier function, junction remodeling, and the molecular architecture of epithelial tissues.
Alterations in PILT expression have been implicated in disease processes such as cancer, inflammation, and developmental abnormalities, where epithelial barrier dysfunction is a contributing factor. Studies also suggest roles for PILT in receptor trafficking and other intracellular pathways, expanding its relevance beyond junctional maintenance. Using a PILT antibody enables exploration of its roles in both healthy tissue physiology and pathological conditions.
NSJ Bioreagents provides a high-quality PILT antibody validated for applications such as western blot, immunofluorescence, and immunohistochemistry. Selecting a PILT antibody from NSJ Bioreagents ensures consistent results and dependable detection in studies of tight junction biology, epithelial polarity, and disease research.
Tight junction-associated protein 1, also called 'Protein incorporated later into tight junctions' (PILT), is a protein that in humans is encoded by the TJAP1 gene. This gene encodes a tight junction-associated protein. Incorporation of the encoded protein into tight junctions occurs at a late stage of formation of the junctions. The encoded protein localizes to the Golgi and may function in vesicle trafficking. Alternatively spliced transcript variants have been described. A related pseudogene exists on the X chromosome.
Optimal dilution of the PILT antibody should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids K160-N557) was used as the immunogen for the PILT antibody.
After reconstitution, the PILT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
