• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> PI3KC3 Antibody

PI3KC3 Antibody (F54724)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F54724-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54724-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
Western blot testing of human HeLa cell lysate with PI3KC3 antibody. Predicted molecular weight ~102 kDa.
›
Western blot testing of human HeLa cell lysate with PI3KC3 antibody. Predicted molecular weight ~102 kDa.
IHC testing of FFPE human liver tissue with PI3KC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human skeletal muscle tissue with PI3KC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with PI3KC3 antibody (green) and Hoechst 33342 nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q8NEB9
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Limitations This PI3KC3 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PI3KC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 14-39 from the human protein was used as the immunogen for the PI3KC3 antibody.

Storage

Aliquot the PI3KC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all PI3KC3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.