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Home >> Antibodies >> PI16 Antibody

PI16 Antibody (RQ6148)

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  Catalog No Formulation Size Price (USD)  
Image RQ6148 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human testicular cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human testicular cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) HeLa, 2) human Jurkat, 3) human PC-3, 4) monkey heart, 5) rat heart, 6) rat testis and 7) mouse heart lysate with PI16 antibody. Predicted molecular weight ~50 kDa, routinely observed at 70-110 kDa depending on glycosylation level.
Flow cytometry testing of human A431 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q6UXB8
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PI16 antibody is available for research use only.
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Description

Peptidase inhibitor 16 is a protein that in humans is encoded by the PI16 gene. PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator.

Application Notes

Optimal dilution of the PI16 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids L28-L363) was used as the immunogen for the PI16 antibody.

Storage

After reconstitution, the PI16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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