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Home >> Antibodies >> Phosphotyrosine Antibody / P-Tyr

Phosphotyrosine Antibody / P-Tyr [clone PY265] (V8317)

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  Catalog No Formulation Size Price (USD)  
Image V8317-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V8317-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V8317SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY265) as confirmation of integrity and purity.
SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY265) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Phosphotyrosine
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PY265
Purity Protein G affinity chromatography
Applications Flow Cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western Blot : 1-2ug/ml
Limitations This Phosphotyrosine antibody is available for research use only.
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Description

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Application Notes

Optimal dilution of the Phosphotyrosine antibody should be determined by the researcher.

Immunogen

Phosphotyrosine was used as the immunogen for the Phosphotyrosine antibody.

Storage

Store the Phosphotyrosine antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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