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Home >> Antibodies >> Phosphorylated JNK Antibody / pThr183/pTyr185

Phosphorylated JNK Antibody / pThr183/pTyr185 [clone RM464] (R20476)

  Catalog No Formulation Size Price (USD)  
Image R20476-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon tissue with recombinant phosphorylated JNK antibody at 1:100.
IHC staining of FFPE human colon cancer tissue with recombinant phosphorylated JNK antibody at 1:100.
Western blot testing of the lysate of human HeLa cells treated (+) or nontreated (-) with UV, using recombinant phosphorylated JNK antibody at a 1:200 dilution.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM464
Purity Protein A purified from animal origin-free supernatant
UniProt P45983 , P45984 , P53779
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Western blot : 1:200-1:400
Limitations This recombinant phosphorylated JNK antibody is available for research use only.
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Description

This antibody reacts to human JNK1, JNK2, and JNK3 only when phosphorylated at Thr183 and Tyr185.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant phosphorylated JNK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A phospho-peptide corresponding to human Phospho-JNK1/2/3 (Thr183/Tyr185) was used as the immunogen for the recombinant phosphorylated JNK antibody.

Storage

Store the recombinant phosphorylated JNK antibody at -20oC.

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