Phosphoprotein 65 Antibody / UL83 [clone rCMV/12688] (V6053)

Immunohistochemistry of Phosphoprotein 65 antibody in human CMV-infected tissue. FFPE human cytomegalovirus-infected tissue demonstrates focal cytoplasmic HRP-DAB brown staining in scattered infected cells, consistent with UL83 / pp65 expression during active viral infection. Clone rCMV/12688, a recombinant mouse monoclonal antibody, was used for detection. An inset image shows PBS substituted for the primary antibody as a negative control, confirming absence of specific staining. Heat-induced epitope retrieval was performed by heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.