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Home >> Antibodies >> Phospho-YAP1 (Ser127) Antibody / Hippo Pathway Activity Marker Antibody

Phospho-YAP1 (Ser127) Antibody / Hippo Pathway Activity Marker Antibody (F48658)

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  Catalog No Formulation Size Price (USD)  
Image F48658-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48658-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Phospho-YAP1 (Ser127) Antibody Dot Blot. Dot blot analysis demonstrates the phospho-specificity of Phospho-YAP1 (Ser127) Antibody. Fifty nanograms of phosphorylated peptide or nonphosphorylated peptide were spotted per dot and probed with the antibody. A strong signal was observed with the phospho peptide, whereas no reactivity was detected with the corresponding nonphosphorylated peptide, confirming selective recognition of YAP1 phosphorylated at serine 127. Phosphorylation of Yes Associated Protein 1 at Ser127 is a hallmark of Hippo pathway activation and promotes cytoplasmic sequestration of YAP1. These results support the utility of Phospho-YAP1 (Ser127) Antibody for monitoring Hippo pathway activity and distinguishing phosphorylated YAP1 from total protein.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Chicken, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P46937
Applications Dot Blot : 1:500
Limitations This Phospho-YAP1 (Ser127) Antibody / Hippo Pathway Activity Marker Antibody is available for research use only.
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Description

Phospho-YAP1 (Ser127) Antibody recognizes Yes Associated Protein 1 phosphorylated at serine 127, a critical regulatory site controlling YAP1 activity and subcellular localization. YAP1 is a transcriptional coactivator and major downstream effector of the Hippo signaling pathway. In response to activation of Hippo kinases, large tumor suppressor kinases phosphorylate YAP1 at Ser127, creating a binding site for 14-3-3 proteins that promotes cytoplasmic retention and prevents nuclear accumulation. This phosphorylation event inhibits YAP1 mediated transcription and serves as a key mechanism for restricting cell proliferation and maintaining tissue homeostasis.

YAP1 integrates signals from cell density, extracellular matrix stiffness, mechanical stress, and growth factor pathways to regulate organ size, differentiation, and tissue regeneration. The balance between phosphorylated and unphosphorylated YAP1 determines whether cells maintain a quiescent state or initiate growth promoting transcriptional programs. Reduced phosphorylation at Ser127 permits nuclear localization and activation of TEAD transcription factors, resulting in expression of genes involved in proliferation, survival, stemness, and epithelial mesenchymal transition. Consequently, phosphorylation of YAP1 at Ser127 serves as an important indicator of Hippo pathway activity and cellular growth control.

Abnormal regulation of YAP1 phosphorylation has been implicated in numerous diseases, including hepatocellular carcinoma, pancreatic cancer, lung cancer, breast cancer, ovarian cancer, colorectal cancer, and glioblastoma. Decreased Ser127 phosphorylation and increased nuclear YAP1 activity have been associated with tumor progression, metastasis, cancer stem cell maintenance, and resistance to chemotherapy. In addition to oncology, altered YAP1 signaling contributes to fibrosis, cardiovascular disease, and regenerative responses following tissue injury. Measurement of phospho YAP1 provides valuable information regarding pathway activation and therapeutic responses.

Phospho-YAP1 (Ser127) Antibody is useful for investigating Hippo pathway signaling, mechanotransduction, stem cell biology, tissue regeneration, and cancer progression. Detection of YAP1 phosphorylated at serine 127 enables researchers to distinguish inactive cytoplasmic YAP1 from transcriptionally active nuclear YAP1 and provides insight into the dynamic regulation of cellular growth and differentiation. Because Ser127 phosphorylation is one of the best characterized markers of Hippo pathway activation, Phospho-YAP1 (Ser127) Antibody is an important tool for studies of development, disease mechanisms, and targeted therapeutics.

For additional YAP1 antibodies validated for WB, IHC, IF, FACS and IP applications, visit our YAP1 Antibody page featuring monoclonal, recombinant and polyclonal antibodies against this key Hippo pathway effector.

Application Notes

Titration of the Phospho-YAP1 (Ser127) Antibody / Hippo Pathway Activity Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This Phospho-YAP1 (Ser127) Antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS127 of human YAP1.

Storage

Aliquot the Phospho-YAP1 (Ser127) Antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Phospho YAP1 S127 Antibody, pYAP1 Ser127 Antibody, Phosphorylated YAP1 Antibody, Phospho Yes Associated Protein 1 Antibody, YAP1 pS127 Antibody, Phosphorylated YAP1 Ser127 Antibody, Phospho YAP Antibody

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