Phospho-SMAD3 (S208) Antibody / Phosphorylated SMAD3 Antibody (F48449)

Phospho-SMAD3 (S208) Antibody HeLa Cell IF. Confocal immunofluorescence was performed on human HeLa cells using Phospho-SMAD3 (S208) Antibody, followed by an Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody (green). F-actin was counterstained with Alexa Fluor 555-conjugated phalloidin (red). Prominent nuclear fluorescence is observed, consistent with activated phosphorylated SMAD3, while the actin cytoskeleton is clearly delineated by phalloidin staining. These results demonstrate that this Phosphorylated SMAD3 Antibody is suitable for confocal immunofluorescent detection of SMAD3 phosphorylated at serine 208.

Phospho-SMAD3 (S208) Antibody Phospho-Peptide Dot Blot. Dot blot analysis was performed to evaluate the phospho-specificity of Phospho-SMAD3 (S208) Antibody. Equal amounts (50 ng) of phosphorylated SMAD3 (S208) peptide (P-peptide) and the corresponding non-phosphorylated peptide (NP-peptide) were spotted onto the membrane. The antibody specifically recognizes the phosphorylated peptide, while no detectable binding is observed to the non-phosphorylated control. These results demonstrate that this Phosphorylated SMAD3 Antibody exhibits excellent phosphorylation site specificity for serine 208, supporting its use in studies of SMAD3 activation.