• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Phospho-SMAD3 Antibody (pS208)

Phospho-SMAD3 Antibody (pS208) (F48449)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F48449-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48449-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
Bulk quote request
›
Confocal immunofluorescent analysis of phospho-SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Dot blot analysis of phospho-SMAD3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Chicken, Mouse, Pig, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P84022
Applications Immunofluorescence : 1:10-1:100
Dot Blot : 1:500
Limitations This phospho-SMAD3 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : FACS, IF
    Reactivity : Human
    Microvalidated
  • Applications : WB, IF, IHC, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Pig, Chicken
  • Applications : IHC, IF, FACS, WB, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat, Pig, Chicken
    Citation  Citations(1)
  • Applications : WB, ELISA
    Reactivity : Mouse, Rat
  • Applications : WB, ELISA
    Reactivity : Mouse, Rat
  • Applications : WB, ELISA
    Reactivity : Rat
  • Applications : WB
    Reactivity : Human, Mouse, Rat

Description

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Application Notes

Titration of the phospho-SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-SMAD3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS208 of human SMAD3.

Storage

Aliquot the phospho-SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all Phospho-SMAD3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.