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Home >> Antibodies >> Phospho-PARP1 (T368) Antibody

Phospho-PARP1 (T368) Antibody (F48732)

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  Catalog No Formulation Size Price (USD)  
Image F48732-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48732-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Dot blot analysis of Phospho-PARP1 (T368) Antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P09874
Applications Dot Blot : 1:500
Limitations This Phospho-PARP1 (T368) Antibody is available for research use only.
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Description

Phospho-PARP1 (T368) Antibody specifically detects PARP1 phosphorylated at the threonine 368 site. This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Phosphorylation-dependent PARP1 signaling studies may also benefit from our phospho-PARP1 (Ser177) antibody page focused on DNA damage response and chromatin stress pathway regulation.

Application Notes

Titration of the Phospho-PARP1 (T368) Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This Phospho-PARP1 (T368) Antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT368 of human PARP1.

Storage

Aliquot the Phospho-PARP1 (T368) Antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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