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Home >> Antibodies >> Phospho-Oct4 Antibody (pS236)

Phospho-Oct4 Antibody (pS236) (F48631)

  Catalog No Formulation Size Price (USD)  
Image F48631-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48631-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Dot blot analysis of phospho-Oct4 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Mouse, Pig
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q01860
Applications Dot Blot : 1:500
Limitations This phospho-Oct4 antibody is available for research use only.
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Description

OCT4 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Application Notes

Titration of the phospho-Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-Oct4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS236 of human OCT4.

Storage

Aliquot the phospho-Oct4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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