- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Phospho-JAK2 (pTyr1007/pTyr1008) Antibody / JAK-STAT Activation Marker [clone IFE-10] (RQ4763)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ4763 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 449 |
| Availability | 1-2 weeks |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Host | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | IFE-10 |
| Purity | Affinity purified |
| UniProt | O60674 |
| Localization | Nuclear, cytoplasmic |
| Applications | Western Blot : 1:500-1:2000 Immunohistochemistry (FFPE) : 1:50-1:200 Immunofluorescence/Immunocytochemistry : 1:50-1:200 |
| Limitations | This Phospho-JAK2 (pTyr1007/pTyr1008) Antibody / JAK-STAT Activation Marker is available for research use only. |
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Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that plays a central role in cytokine receptor signaling and regulation of immune, hematopoietic, and inflammatory pathways. Activation of JAK2 occurs through phosphorylation of key residues within the activation loop, specifically tyrosine 1007 and tyrosine 1008. Phospho-JAK2 (pTyr1007/pTyr1008) Antibody, clone IFE-10, is designed to detect JAK2 phosphorylated at these sites, providing a direct readout of kinase activation and signaling pathway engagement.
JAK2 is activated downstream of cytokine receptors such as those for interleukins, interferons, and growth factors. Upon ligand binding, receptor-associated JAK2 undergoes trans-phosphorylation at Tyr1007 and Tyr1008, resulting in conformational activation of the kinase domain. This phosphorylation event is essential for catalytic activity and enables phosphorylation of downstream substrates, including STAT transcription factors that mediate gene expression responses.
Phosphorylation at Tyr1007 and Tyr1008 represents a canonical activation event and is widely used as a marker of JAK2 signaling. Unlike total JAK2 detection, which reflects protein expression levels, phospho-specific detection at these residues provides insight into functional activation of the kinase. Increased phosphorylation is typically observed following cytokine stimulation and is associated with activation of the JAK-STAT signaling pathway.
The JAK2-STAT axis is critical for regulating processes such as cell proliferation, differentiation, immune response, and hematopoiesis. Dysregulation of JAK2 signaling is implicated in a variety of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disorders. Mutations such as JAK2 V617F lead to constitutive activation of the kinase and are commonly observed in hematologic malignancies.
Subcellularly, activated JAK2 is localized primarily in the cytoplasm and at the plasma membrane in association with cytokine receptors. Upon activation, downstream STAT proteins are phosphorylated and translocate to the nucleus to regulate gene expression. Detection of JAK2 phosphorylation therefore provides an upstream marker of pathway activation that precedes STAT signaling events.
Phospho-JAK2 (pTyr1007/pTyr1008) Antibody, clone IFE-10, enables selective detection of activated JAK2, supporting studies of cytokine signaling, immune regulation, and kinase-driven disease mechanisms. Its specificity for activation loop phosphorylation makes it a valuable tool for assessing JAK-STAT pathway activity in a wide range of biological systems. This antibody is part of our full phospho antibody collection which can be explored for additional phosphorylation-specific targets and pathway markers.
Optimal dilution of the Phospho-JAK2 (pTyr1007/pTyr1008) Antibody / JAK-STAT Activation Marker should be determined by the researcher.
A synthetic peptide specific to human JAK2 (surrounding pY1007/pY1008) was used as the immunogen for the Phospho-JAK2 (pTyr1007/pTyr1008) Antibody.
Store the Phospho-JAK2 (pTyr1007/pTyr1008) Antibody at -20oC.
Phospho JAK2 antibody, JAK2 pTyr1007 antibody, JAK2 pTyr1008 antibody, JAK2 Tyr1007 1008 antibody, JAK2 phospho antibody, phosphorylated JAK2 antibody, JAK2 activation loop antibody, JAK STAT signaling antibody, cytokine signaling JAK2 antibody, clone IFE-10 antibody
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