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Home >> Antibodies >> Phospho-Histone H3 Antibody (pS10)

Phospho-Histone H3 Antibody (pS10) (F48401)

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  Catalog No Formulation Size Price (USD)  
Image F48401-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
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F48401-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody
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Western blot analysis of lysate from HeLa cell line, untreated or treated with Calyculin A (100ng/ml), using phospho-Histone H3 antibody
Western blot analysis of lysate from HeLa cell line, untreated or treated with FBS + Calyculin A (100ng/ml), using phospho-Histone H3 antibody
Western blot analysis of lysate from HeLa cell line using phospho-Histone H3 antibody at 1:1000.
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS + Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
Western blot analysis of extracts from HeLa cells, untreated or treated with FBS and Calyculin A (100nM), using phospho-Histone H3 antibody (left) or nonphos Ab (right).
Dot blot analysis of phospho-Histone H3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
IHC staining of FFPE human tonsil with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with phospho-Histone H3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA fixed and permeabilized human HeLa cells with phospho-Histone H3 antibody (green) and Phalloidin (red).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P68431
Applications Western blot : 1:500-4000
Immunohistochemistry (FFPE) : 1:-50-1:100
Immunofluorescence : 1:25
Dot blot : 1:500
Limitations This phospho-Histone H3 antibody is available for research use only.
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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Application Notes

Titration of the phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-Histone H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS10 of human HIST1H3A.

Storage

Aliquot the phospho-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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