Phospho-FOXO3A (S253) Antibody / Phosphorylated FOXO3A Antibody [clone ECG-6] (RQ4742)

Phospho-FOXO3A (S253) Antibody IGF-Stimulated MCF-7 WB. Western blot analysis was performed using Phospho-FOXO3A (S253) Antibody on IGF-treated human MCF-7 cell lysate. A distinct immunoreactive band is detected at approximately 90 kDa, consistent with the expected migration of phosphorylated FOXO3A, which is commonly observed between 71–90 kDa depending on glycosylation and other post-translational modifications. Detection following IGF stimulation reflects activation of the PI3K/AKT signaling pathway and phosphorylation of FOXO3A at serine 253. This Phospho-FOXO3A (S253) Antibody / Phosphorylated FOXO3A Antibody is well suited for Western blot studies of AKT signaling, growth factor responses, FOXO3A regulation, and phosphorylation-dependent transcriptional control.

Phospho-FOXO3A (S253) Antibody Multi-Species Cell and Brain WB. Western blot analysis was performed using Phospho-FOXO3A (S253) Antibody on lane 1: human T47D, lane 2: human K562, lane 3: human Caco-2, lane 4: human PC-3, lane 5: rat brain, lane 6: rat C6, lane 7: mouse brain, and lane 8: mouse Neuro-2a lysates. A prominent immunoreactive band is detected at approximately 80–100 kDa across all samples, consistent with the expected migration of phosphorylated FOXO3A, which commonly migrates above its predicted molecular weight due to post-translational modifications. This Phospho-FOXO3A (S253) Antibody / Phosphorylated FOXO3A Antibody is well suited for Western blot studies of AKT signaling, FOXO3A phosphorylation, growth factor-mediated signaling, and phosphorylation-dependent transcriptional regulation.

Phospho-FOXO3A (S253) Antibody Human Uterine Cancer IHC. Phospho-FOXO3A (S253) Antibody was used for immunohistochemical staining of FFPE human uterine cancer tissue. Following antigen retrieval, the tissue was incubated with the phospho-specific primary antibody, followed by an HRP-conjugated secondary antibody and chromogenic detection. Predominantly cytoplasmic staining is observed in the malignant epithelial cells, consistent with phosphorylation of FOXO3A at serine 253, an event that promotes nuclear export and transcriptional inactivation through AKT signaling. This Phospho-FOXO3A (S253) Antibody / Phosphorylated FOXO3A Antibody is well suited for investigating PI3K/AKT pathway activation, FOXO3A phosphorylation, growth factor signaling, and phosphorylation-dependent regulation of tumor biology.