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Home >> Antibodies >> Phospho-ER alpha Antibody (pS118)

Phospho-ER alpha Antibody (pS118) [clone EIG-5] (RQ5023)

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Image RQ5023 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
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Western blot testing of human MCF7 cells 1) untreated and 2) treated with b-Estradiol and EGF, with phospho-ER alpha antibody. Predicted molecular weight ~66 kDa.
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Western blot testing of human MCF7 cells 1) untreated and 2) treated with b-Estradiol and EGF, with phospho-ER alpha antibody. Predicted molecular weight ~66 kDa.
IHC staining of FFPE human kidney tissue with phospho-ER alpha antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with phospho-ER alpha antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with phospho-ER alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostatic hyperplasia tissue with phospho-ER alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat uterus tissue with phospho-ER alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat C6, 3) mouse brain and 4) mouse Neuro-2a cell lysate with phospho-ER alpha antibody. Predicted molecular weight ~66 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name EIG-5
Purity Affinity purified
UniProt P03372
Applications Western Blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This phospho-ER alpha antibody is available for research use only.
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Description

Estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. [RefSeq]

Application Notes

Optimal dilution of the phospho-ER alpha antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human ER alpha / ESR1 (surrounding pS118) was used as the immunogen for the phospho-ER alpha antibody.

Storage

Store the phospho-ER alpha antibody at -20oC.

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