Phospho-ER alpha (pS118) Antibody / Hormone-Induced Activation Marker Antibody [clone EIG-5] (RQ5023)

Phospho-ER alpha (pS118) Antibody MCF7 WB. Western blot analysis of human MCF7 cell lysates (lane 1: untreated, lane 2: beta-estradiol and EGF treated) using phospho-ER alpha (pS118) antibody detects a band at approximately 65-70 kDa, consistent with the predicted molecular weight of Estrogen receptor alpha (ESR1), with increased signal in treated cells reflecting hormone- and growth factor-induced phosphorylation; clone EIG-5 was used for detection.

Phospho-ER alpha (pS118) Antibody Kidney Tissue IHC. Immunohistochemistry of FFPE human kidney tissue using phospho-ER alpha (pS118) antibody shows HRP-DAB brown nuclear staining in epithelial cells, consistent with phosphorylation at serine 118 and activation of ESR1 signaling, while surrounding stromal elements display lower background; detection was performed with clone EIG-5. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Phospho-ER alpha (pS118) Antibody Kidney Tissue IHC. Immunohistochemistry of FFPE human kidney tissue using phospho-ER alpha (pS118) antibody shows HRP-DAB brown nuclear staining in epithelial cells, consistent with phosphorylation at serine 118 and activation of ESR1 signaling, while surrounding stromal elements display lower background; detection was performed with clone EIG-5. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Phospho-ER alpha (pS118) Antibody Breast Cancer Tissue IHC. Immunohistochemistry of FFPE human breast carcinoma tissue using phospho-ER alpha (pS118) antibody shows prominent HRP-DAB brown nuclear staining in tumor epithelial cells, consistent with hormone- and growth factor-induced phosphorylation at serine 118 and active ESR1 signaling, while surrounding stromal cells display lower background; detection was performed with clone EIG-5. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-ER alpha (pS118) Antibody Prostatic Hyperplasia IHC. Immunohistochemistry of FFPE human prostatic hyperplasia tissue using phospho-ER alpha (pS118) antibody shows HRP-DAB brown nuclear staining in glandular epithelial cells, consistent with phosphorylation at serine 118 and hormonally regulated ESR1 activation, while surrounding stromal elements display lower background; detection was performed with clone EIG-5. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-ER alpha (pS118) Antibody Rat Uterus IHC. Immunohistochemistry of FFPE rat uterus tissue using phospho-ER alpha (pS118) antibody demonstrates HRP-DAB brown nuclear staining in uterine epithelial and stromal cells, consistent with estrogen-responsive phosphorylation at serine 118 and active ESR1 signaling in reproductive tissue, while background staining remains minimal; detection was performed with clone EIG-5. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-ER alpha (pS118) Antibody Mouse Rat WB. Western blot testing of 1) rat brain, 2) rat C6, 3) mouse brain and 4) mouse Neuro-2a cell lysate with phospho-ER alpha antibody. Predicted molecular weight ~66 kDa.