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Home >> Antibodies >> Phospho-CDK1/2 Antibody (pT14)

Phospho-CDK1/2 Antibody (pT14) [clone DFH-3] (RQ5152)

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Image RQ5152 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of lysate from human HeLa cells treated with hydroxyurea, with phospho-CDK1/2 antibody (pT14). Predicted molecular weight ~33 kDa.
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Western blot testing of lysate from human HeLa cells treated with hydroxyurea, with phospho-CDK1/2 antibody (pT14). Predicted molecular weight ~33 kDa.
IHC staining of FFPE rat spleen tissue with phospho-CDK1/2 antibody (pT14). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DFH-3
Purity Affinity purified
UniProt P06493
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This phospho-CDK1/2 antibody (pT14) is available for research use only.
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Description

Cyclin-dependent kinases (CDKs) are a family of protein kinases first discovered for their role in regulating the cell cycle. They are also involved in regulating transcription, mRNA processing, and the differentiation of nerve cells.[1] They are present in all known eukaryotes, and their regulatory function in the cell cycle has been evolutionarily conserved. [Wiki]

Application Notes

Optimal dilution of the phospho-CDK1/2 antibody (pT14) should be determined by the researcher.

Immunogen

A synthetic peptide specific to human CDK1/2 (surrounding phosphorylated T14) was used as the immunogen for the phospho-CDK1/2 antibody.

Storage

Store the phospho-CDK1/2 antibody (pT14) at -20oC.

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