Phospho-AKT1 (pT450) Antibody / Activation and PI3K-AKT Signaling Marker Antibody [clone HEA-1] (RQ8829)

Phospho-AKT1 (pT450) Antibody Rat Colon WB. Western blot analysis of rat colon tissue lysates under normal, control, low-dose, medium-dose, and positive treatment conditions shows a band at approximately 70 kDa using phospho-AKT1 (pT450) antibody, compared to the predicted molecular weight of ~56 kDa for AKT1, consistent with phosphorylation-dependent mobility shift and activation state of the kinase; signal intensity varies across treatment groups, reflecting modulation of AKT1 phosphorylation, while ACTIN serves as a loading control; clone HEA-1 was used for detection.

Phospho-AKT1 (pT450) Antibody Lymphoma Tissue IHC. Immunohistochemistry of FFPE human intestinal diffuse large B-cell lymphoma tissue using phospho-AKT1 (pT450) antibody shows HRP-DAB brown cytoplasmic and nuclear staining in malignant lymphoid cells, consistent with activated PI3K-AKT signaling and phosphorylation at the T450 turn motif, while surrounding stromal elements display lower background; clone HEA-1 was used for detection. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-AKT1 (pT450) Antibody Lung Adenocarcinoma IHC. Immunohistochemistry of FFPE human lung adenocarcinoma tissue using phospho-AKT1 (pT450) antibody demonstrates HRP-DAB brown cytoplasmic and nuclear staining in tumor epithelial cells, consistent with phosphorylation-dependent AKT1 activation within PI3K-AKT signaling, while stromal regions show comparatively reduced staining; detection was performed with clone HEA-1. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-AKT1 (pT450) Antibody Prostate Cancer Tissue IHC. Immunohistochemistry of FFPE human prostate carcinoma tissue using phospho-AKT1 (pT450) antibody shows HRP-DAB brown cytoplasmic and nuclear staining in tumor epithelial cells, consistent with phosphorylation at the T450 turn motif and active PI3K-AKT signaling, while surrounding stromal components display lower background; detection was performed with clone HEA-1. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-AKT1 (pT450) Antibody Colorectal Adenocarcinoma IHC. Immunohistochemistry of FFPE human colorectal adenocarcinoma tissue using phospho-AKT1 (pT450) antibody shows HRP-DAB brown cytoplasmic and nuclear staining in tumor epithelial cells, consistent with phosphorylation at the T450 turn motif and active PI3K-AKT signaling, while adjacent stromal regions display lower background; detection was performed with clone HEA-1. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Phospho-AKT1 (pT450) Antibody WB. Western blot testing of 1) human HeLa, 2) human MCF7, 3) rat brain, 4) rat PC-12, 5) mouse brain and 6) mouse NIH 3T3 cell lysate with Phospho-AKT1 Antibody.

Phospho-AKT1 (pT450) Antibody A549 IF. Immunofluorescence analysis of FFPE human A549 cells using phospho-AKT1 (pT450) antibody (green) shows cytoplasmic and nuclear signal consistent with phosphorylation at the T450 turn motif, while Beta Actin (red) highlights the cytoskeletal network; nuclei are counterstained with DAPI (blue). Detection was performed with clone HEA-1. HIER: steam sections in pH6 citrate buffer for 20 min.