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Home >> Antibodies >> Phospho-AKT Antibody (pS473)

Phospho-AKT Antibody (pS473) (F48521)

  Catalog No Formulation Size Price (USD)  
Image F48521-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 389
Image
F48521-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot analysis of extracts from NIH3T3 cells, untreated or treated with PDGF, using phospho-AKT antibody (left) or nonphos-AKT antibody (right).
Dot blot analysis of phospho-AKT antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P31749
Applications Dot blot : 1:500
Western blot : 1:1000
Limitations This phospho-AKT antibody is available for research use only.
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Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Application Notes

Titration of the phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-AKT antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS473 of human AKT1.

Storage

Aliquot the phospho-AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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