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Home >> Antibodies >> PHGDH Antibody

PHGDH Antibody (F48339)

  Catalog No Formulation Size Price (USD)  
Image F48339-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F48339-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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PHGDH antibody western blot analysis in Ramos lysate. Predicted molecular weight ~56 kDa.
PHGDH antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
PHGDH antibody flow cytometric analysis of Ramos cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Primate, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O43175
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This PHGDH antibody is available for research use only.
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Description

3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

Application Notes

Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 249-277 from the human protein was used as the immunogen for this PHGDH antibody.

Storage

Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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