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Home >> Antibodies >> PHGDH Antibody

PHGDH Antibody (F48338)

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  Catalog No Formulation Size Price (USD)  
Image F48338-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48338-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.
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Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.
Western blot analysis of PHGDH antibody and mouse brain tissue lysate. Predicted molecular weight ~56 kDa.
IHC analysis of FFPE human brain tissue stained with PHGDH antibody
Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
  • Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.
  • Western blot analysis of PHGDH antibody and mouse brain tissue lysate. Predicted molecular weight ~56 kDa.
  • IHC analysis of FFPE human brain tissue stained with PHGDH antibody
  • Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a <a href=negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." title="Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis." onClick="showhideiamges('image4','imagelargedata4',4,4)">
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Bovine, Pig, Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O43175
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This PHGDH antibody is available for research use only.
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Description

3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

Application Notes

Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 140-168 from the human protein was used as the immunogen for this PHGDH antibody.

Storage

Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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