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Home >> Antibodies >> PHF6 Antibody / PHD finger protein 6

PHF6 Antibody / PHD finger protein 6 (RQ8782)

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  Catalog No Formulation Size Price (USD)  
Image RQ8782 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A549 cells with PHF6 antibody (red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with PHF6 antibody (red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human pancreas ductal adenocarcinoma tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreas ductal adenocarcinoma tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human mouse brain tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with PHF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human 293T, 2) human Jurkat, 3) human HeLa, 4) monkey COS-7, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with PHF6 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of fixed and permeabilized human JK cells with PHF6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHF6 antibody.
Availability 1-3 days
Species Reactivity Human, Monkey, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q8IWS0
Localization Nuclear
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This PHF6 antibody is available for research use only.
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Description

PHD finger protein 6 is a protein that in humans is encoded by the PHF6 gene. This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by cognitive disability, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate splicing results in multiple transcript variants, encoding different isoforms.

Application Notes

Optimal dilution of the PHF6 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids Q37-D309) was used as the immunogen for the PHF6 antibody.

Storage

After reconstitution, the PHF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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