PHF23 Antibody / PHD finger protein 23 Antibody (FY13544)

PHF23 Antibody by IF in mouse brain tissue. Immunofluorescence analysis using PHD finger protein 23 Antibody demonstrates punctate red PHF23-associated staining among densely distributed blue DAPI-stained nuclei in a paraffin-embedded mouse brain section. Antigen retrieval was performed using heated EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. The primary antibody was incubated at 5 ug/mL overnight at 4oC and detected with a Cy3-conjugated anti-rabbit secondary antibody at 1:500. The merged fluorescence image shows the distribution of PHF23-associated signal relative to nuclear counterstaining.

PHF23 Antibody by IHC in mouse brain at low magnification. Immunohistochemistry using PHD finger protein 23 Antibody on paraffin-embedded mouse brain demonstrates light brown immunoreactivity distributed across the tissue, with prominent anatomical layering and hematoxylin-stained nuclei. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC. An HRP-conjugated anti-rabbit secondary antibody and DAB chromogen were used to visualize staining. The overview preserves the regional organization of the brain section.

PHF23 Antibody by IF in A549 cells. Immunofluorescence analysis using PHD finger protein 23 Antibody demonstrates green fluorescence in A549 cells, predominantly around the blue nuclear counterstain and in adjacent cellular regions. Cells underwent enzyme-mediated antigen retrieval, blocking with 10% goat serum and incubation with primary antibody at 5 ug/mL overnight at 4oC. A fluorescent anti-rabbit secondary antibody was applied at 1:500 before imaging. The merged image provides a cellular view of PHF23-associated staining, with individual blue nuclei distinguishable from surrounding green fluorescence.

PHF23 Antibody by WB in rodent brain. Western blot analysis using PHD finger protein 23 Antibody detects a principal band near 65 kDa in rat brain (lane 1) and mouse brain (lane 2), consistent with the expected migration reported for PHF23. Reduced tissue lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was visualized using an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate. Both lanes demonstrate PHF23-associated reactivity at a comparable molecular weight.

PHF23 Antibody by IHC in mouse brain at higher magnification. Immunohistochemistry of paraffin-embedded mouse brain using PHD finger protein 23 Antibody shows brown staining in numerous cell nuclei, with blue hematoxylin counterstaining providing contrast among neighboring cells. Sections underwent EDTA-based heat-mediated antigen retrieval at pH 8.0 and blocking with 10% goat serum. The primary antibody was applied at 2 ug/mL overnight at 4oC, followed by an HRP-conjugated anti-rabbit secondary antibody and DAB development. This field provides a closer view of PHF23-associated staining within mouse brain tissue.

PHF23 Antibody by IHC in rat brain. Immunohistochemistry of paraffin-embedded rat brain using PHD finger protein 23 Antibody shows variable nuclear brown immunoreactivity among cells in the examined tissue, with hematoxylin highlighting the surrounding nuclei. Heat-mediated antigen retrieval was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC and developed with an HRP-conjugated anti-rabbit secondary antibody and DAB chromogen. The image documents PHF23-associated immunoreactivity in rat brain.

PHF23 Antibody by FACS in 293T cells. Flow cytometry analysis using PHD finger protein 23 Antibody evaluates intracellular staining in fixed, permeabilized 293T cells. The blue antibody-stained fluorescence distribution is compared with green isotype and red unstained controls. Cells were blocked with normal goat serum and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by a fluorescent anti-rabbit secondary antibody. The overlay histogram demonstrates a fluorescence shift associated with PHF23 antibody staining relative to the assay controls.