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Home >> Antibodies >> PHB Antibody / Prohibitin

PHB Antibody / Prohibitin [clone PBTN-1] (V7598)

  Catalog No Formulation Size Price (USD)  
Image V7598-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7598-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7598SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7598IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human liver tissue with PHB antibody (clone PBTN-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescence staining of human HepG2 cells with PHB antibody (green, clone PBTN-1) and Reddot nuclear stain (red).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PBTN-1
Purity Protein G affinity chromatography
UniProt P35232
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This PHB antibody is available for research use only.
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Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Ant-iproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. [RefSeq]

Application Notes

The stated application concentrations are suggested starting points. Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Storage

Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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