PGRMC2 Antibody / Progesterone receptor membrane component 2 Antibody (FY13534)

PGRMC2 Antibody by IF in HeLa cells. Immunofluorescence staining of human HeLa cells with Progesterone receptor membrane component 2 Antibody shows green fluorescence predominantly in the cytoplasmic compartment surrounding DAPI-stained blue nuclei. Cells underwent enzyme-mediated antigen retrieval for 15 minutes, were blocked with 10% goat serum, and incubated with primary antibody at 5 ug/mL overnight at 4oC. DyLight 488-conjugated secondary antibody was used at 1:500 for 30 minutes at 37oC. The image demonstrates cellular PGRMC2-associated immunoreactivity with nuclear counterstaining.

PGRMC2 Antibody by IHC in human endometrial carcinoma. Immunohistochemistry of paraffin-embedded human endometrial carcinoma using Progesterone receptor membrane component 2 Antibody reveals brown immunoreactivity in glandular tumor cells, with hematoxylin-counterstained nuclei. Sections underwent EDTA-based antigen retrieval at pH 8.0 and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary detection and DAB development. The image illustrates PGRMC2-associated staining in endometrial tumor epithelium.

PGRMC2 Antibody by IF in human endometrial carcinoma. Immunofluorescence staining of a paraffin-embedded human endometrial carcinoma section with Progesterone receptor membrane component 2 Antibody shows red fluorescence in glandular tumor epithelium and blue DAPI-stained nuclei. Heat-mediated retrieval used EDTA buffer at pH 8.0. Following blocking with 10% goat serum, sections were incubated with primary antibody at 5 ug/mL overnight at 4oC. Cy3-conjugated secondary antibody was applied at 1:500 for 30 minutes at 37oC. The staining pattern demonstrates PGRMC2-associated signal in the tumor tissue.

PGRMC2 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of paraffin-embedded human thyroid carcinoma using Progesterone receptor membrane component 2 Antibody shows brown staining in cells surrounding follicle-like spaces, with blue hematoxylin nuclear counterstaining. EDTA buffer at pH 8.0 was used for heat-mediated antigen retrieval, followed by 10% goat serum blocking. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC and developed with a peroxidase-conjugated secondary reagent and DAB. This image provides additional tissue evidence of PGRMC2-associated immunoreactivity.

PGRMC2 Antibody by WB in human and rodent lysates. Western blot analysis using Progesterone receptor membrane component 2 Antibody detects bands near 24-28 kDa in HeLa (lane 1), HepG2 (lane 2), MCF-7 (lane 3), SK-OV-3 (lane 4), rat liver (lane 5), rat heart (lane 6), and mouse liver (lane 7). This migration is broadly consistent with the calculated PGRMC2 mass of 23.8 kDa. Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. Primary antibody was incubated at 0.5 ug/mL overnight at 4oC, followed by HRP-based chemiluminescent detection.