PGP9.5 Antibody / Microarray Specificity Validated Antibody [clone UCHL1/4558] (V9290)

PGP9.5 Antibody Microarray Specificity Validated. Analysis of human protein microarray (HuProt). Human proteome microarray screening using PGP9.5 Antibody Microarray Specificity Validated, also known as UCHL1 antibody or Ubiquitin C-terminal hydrolase L1 antibody, demonstrates highly selective binding to UCHL1 as the top-ranked target among more than 19,000 full-length human proteins. Clone UCHL1/4558 shows a dominant Z-score and strong S-score separation from all non-target proteins, confirming exceptional specificity and minimal cross-reactivity. Z-score reflects signal intensity relative to the array mean, while S-score represents the specificity gap between the top target and subsequent proteins, supporting high-confidence target recognition in complex proteomic environments.

PGP9.5 Antibody for WB. Western blot analysis in human brain and T98G cell lysates. Human brain tissue and T98G cell lysates stained with PGP9.5 Antibody for WB, also known as Microarray Specificity Validated Antibody and Ubiquitin C-terminal hydrolase L1 antibody, show clear bands at approximately 25 kDa, consistent with the predicted molecular weight of PGP9.5 / UCHL1. The clean, well-defined bands across both lysates support highly specific detection of UCHL1, aligning with proteome-scale microarray validation demonstrating minimal cross-reactivity.

PGP9.5 Antibody for IHC. Immunohistochemistry analysis of PGP9.5 / UCHL1 in human cerebellum tissue. FFPE human cerebellum stained with PGP9.5 Antibody for IHC, also known as Microarray Specificity Validated Antibody and Ubiquitin C-terminal hydrolase L1 antibody, shows strong cytoplasmic and neuropil-associated HRP-DAB brown staining in neuronal cells, consistent with the known neuronal enrichment of UCHL1. The clean staining pattern with low background supports highly specific target recognition, aligning with proteome-scale microarray validation demonstrating minimal cross-reactivity in complex tissue environments.

IHC staining of FFPE human cerebellum tissue with PGP9.5 antibody (clone UCHL1/4558). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PGP9.5 antibody (clone UCHL1/4558) as confirmation of integrity and purity.