PGP9.5 Antibody Clone 31A3 / UCHL1 Antibody [clone 31A3] (V2293)

Description

Protein gene product 9.5 (PGP9.5), also known as Ubiquitin C-terminal hydrolase L1 (UCHL1), is a cytoplasmic protein commonly used as a neuronal and tumor-associated marker. The PGP9.5 Antibody Clone 31A3 is a widely cited monoclonal antibody that has been used in numerous peer-reviewed studies, making it a recognized reference clone for detecting UCHL1 across diverse research applications.

PGP9.5 Antibody Clone 31A3, also referred to as UCHL1 antibody or ubiquitin C-terminal hydrolase L1 antibody, is frequently selected based on literature precedence and established clone performance. Clone 31A3 antibody is consistently reported in published research, supporting its role as a trusted and reproducible reagent. Its repeated use across independent studies allows researchers to align new findings with previously published data, which is particularly important for comparative and longitudinal analysis.

This mouse monoclonal PGP9.5 Antibody Clone 31A3 provides reliable detection of UCHL1 in research applications where clone consistency and literature continuity are key considerations. The use of a well-cited antibody clone reduces variability between studies and supports reproducibility across laboratories. PGP9.5 Antibody Clone 31A3 is therefore especially valuable for researchers seeking a standardized tool that aligns with established experimental results.

UCHL1 is involved in ubiquitin-mediated protein regulation and is expressed in neuronal tissues as well as various cancers. Its expression patterns are frequently analyzed in studies of differentiation, disease progression, and cellular signaling. Detection of UCHL1 using a reference clone such as PGP9.5 Antibody Clone 31A3 enables more direct comparison of expression data across studies and experimental systems.

A PGP9.5 Antibody Clone 31A3 is ideally suited for research applications where a publication-supported, widely used antibody clone is preferred. Its strong presence in the scientific literature distinguishes it from less established reagents and positions it as a dependable choice for reproducible detection of UCHL1.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Native protein from brain was used as the immunogen for this PGP9.5 antibody.

Storage

Store the PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

UCHL1 antibody, PGP9.5 protein antibody, Ubiquitin C-terminal hydrolase L1 antibody, Clone 31A3 antibody