PGP9.5 Antibody / UchL1 [clone 31A3] (V2293)

Description

PGP9.5 Antibody / UchL1 targets ubiquitin C-terminal hydrolase L1, a cytoplasmic protein historically identified as Protein gene product 9.5. This protein is a member of the ubiquitin carboxyl-terminal hydrolase family and plays an essential role in the ubiquitin-proteasome system by cleaving ubiquitin from small adducts and precursor proteins. Through this activity, it supports ubiquitin recycling and contributes to maintenance of protein homeostasis, particularly in cells with high metabolic and synaptic demands.

PGP9.5 is most prominently expressed in neurons of the central and peripheral nervous systems, where it serves as a well-established pan-neuronal marker. In neural tissue, the protein localizes predominantly to the cytoplasm of neuronal cell bodies and extends into axons and dendrites, reflecting its association with neuronal processes. As a result, immunohistochemical detection using a PGP9.5 antibody is widely applied to visualize neuronal architecture, nerve fibers, and peripheral innervation in complex tissues.

Ubiquitin C-terminal hydrolase L1, commonly referred to as UchL1, contributes to neuronal integrity by regulating ubiquitin availability and influencing proteasome-dependent degradation pathways. Proper function of this enzyme is important for synaptic maintenance and axonal stability, and alterations in UchL1 expression or activity have been linked to neurodegenerative disease mechanisms. Associations have been reported with conditions such as Parkinson disease and other disorders characterized by abnormal protein aggregation, highlighting the biological relevance of UchL1 beyond its utility as a histological marker.

In addition to its role in the nervous system, UchL1 expression has been documented in neuroendocrine cells and in a range of tumors, particularly those exhibiting neural or neuroendocrine differentiation. In these contexts, PGP9.5 Antibody / UchL1 is frequently used in immunohistochemistry to identify nerve fiber involvement, neuronal differentiation, or neural elements within tumor tissue and surrounding stroma. Its characteristic staining pattern in formalin-fixed, paraffin-embedded specimens has made PGP9.5 one of the most commonly used neuronal markers in both research and diagnostic pathology settings.

PGP9.5 Antibody / UchL1 (clone 31A3) is designed for detection of UchL1 in research applications. In immunohistochemical analysis of human brain tissue, staining is typically observed in neuronal cell bodies and associated processes, consistent with the known cytoplasmic localization of the protein. As with other neuronal markers, appropriate antigen retrieval conditions are important for optimal detection in fixed tissues. Overall, UchL1 remains a biologically significant component of ubiquitin-mediated pathways and a robust marker for neuronal identification in neuroscience and pathology research.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Native protein from brain was used as the immunogen for this PGP9.5 antibody.

Storage

Store the PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).