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Home >> Antibodies >> PGP9.5 Antibody

PGP9.5 Antibody [clone UBCE-L1] (V7153)

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  Catalog No Formulation Size Price (USD)  
Image V7153-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7153-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7153SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7153IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE rat cerebellum with PGP9.5 antibody (clone UBCE-L1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE rat cerebellum with PGP9.5 antibody (clone UBCE-L1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human brain lysate with PGP9.5 antibody (clone UBCE-L1). Predicted molecular weight ~25 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name UBCE-L1
Purity Protein G affinity chromatography
UniProt P09936
Localization Cytoplasmic, ER membrane
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This PGP9.5 antibody is available for research use only.
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Description

UCHL1/PGP9.5 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2% of total brain protein), expressed specifically in neurons and testis/ovary. [Wiki]

Application Notes

Optimal dilution of the PGP9.5 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the PGP9.5 antibody.

Storage

Store the PGP9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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