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Home >> Antibodies >> PGP9.5 Antibody

PGP9.5 Antibody (F47952)

  Catalog No Formulation Size Price (USD)  
Image F47952-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F47952-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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PGP9.5 antibody western blot analysis with (1) U266, (2) SH-SY5Y, (3) NCI-H1299, (4) mouse Neuro-2a, (5) rat C6, (6) PC-12 cell line and (7) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
IHC analysis of FFPE human breast carcinoma tissue stained with the PGP9.5 antibody
PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human brain tissue stained with the PGP9.5 antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Rat, Bovine, Pig, Primate
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P09936
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This PGP9.5 antibody is available for research use only.
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Description

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.

Application Notes

Titration of the PGP9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 187-216 from the human protein was used as the immunogen for this PGP9.5 antibody.

Storage

Aliquot the PGP9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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