PGP Antibody / Glycerol-3-phosphate Phosphatase Antibody (RQ8108)

PGP Antibody IF Staining of Human Liver Cancer Tissue. Immunofluorescence analysis of FFPE human liver cancer tissue using Glycerol-3-phosphate Phosphatase Antibody. Red PGP-associated fluorescence is distributed throughout the tissue, with prominent staining in cytoplasmic regions surrounding blue DAPI-stained nuclei. Heat-induced epitope retrieval was performed by steaming sections in EDTA buffer (pH 8) for 20 minutes.

PGP Antibody IF Staining of Human Intestinal Cancer Tissue. Immunofluorescence analysis of FFPE human intestinal cancer tissue using Glycerol-3-phosphate Phosphatase Antibody. Red fluorescence is visible throughout the tissue, including regions surrounding glandular structures, while DAPI counterstaining identifies cell nuclei in blue. Heat-induced epitope retrieval was performed by steaming sections in EDTA buffer (pH 8) for 20 minutes.

PGP Antibody IF Staining of Human PC-3 Cells. Immunofluorescence analysis of FFPE human PC-3 cells using Glycerol-3-phosphate Phosphatase Antibody. Red PGP-associated fluorescence is visible predominantly in cytoplasmic regions surrounding the blue DAPI-stained nuclei. Heat-induced epitope retrieval was performed using citrate buffer (pH 6) for 20 minutes.

PGP Antibody IHC Staining of Human Esophageal Squamous Carcinoma. Immunohistochemical analysis of FFPE human esophageal squamous carcinoma tissue using Glycerol-3-phosphate Phosphatase Antibody. Brown DAB staining is distributed throughout the tumor tissue, with cytoplasmic staining visible in numerous cells and blue hematoxylin nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

PGP Antibody IHC Staining of Human Endometrial Adenocarcinoma. Immunohistochemical analysis of FFPE human endometrial adenocarcinoma tissue using Glycerol-3-phosphate Phosphatase Antibody. Brown DAB staining is prominent in the glandular tumor epithelium, with comparatively lighter staining in portions of the surrounding stromal tissue. Hematoxylin provides blue nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

PGP Antibody IHC Staining of Human Tonsil Tissue. Immunohistochemical analysis of FFPE human tonsil tissue using Glycerol-3-phosphate Phosphatase Antibody. Brown DAB staining is visible in numerous cells throughout the tissue, with heterogeneous distribution and areas of more prominent cytoplasmic staining. Hematoxylin provides blue nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

PGP Antibody IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of FFPE human lung cancer tissue using Glycerol-3-phosphate Phosphatase Antibody. Brown DAB staining is prominent in numerous tumor-associated cells, including cells arranged around glandular and alveolar-like spaces. Staining is predominantly cytoplasmic, with blue hematoxylin nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

PGP Antibody WB Analysis of Human HeLa, MCF7, 293T and Rodent Tissue Lysates. Western blot analysis using Glycerol-3-phosphate Phosphatase Antibody with human HeLa (lane 1), MCF7 (lane 2), and 293T (lane 3) cell lysates, rat brain (lane 4), rat lung (lane 5), mouse brain (lane 6), and mouse lung (lane 7) tissue lysates. A band near 34-36 kDa is detected in multiple samples, consistent with the predicted molecular weight of PGP.

PGP Antibody FACS Analysis of Human PC-3 Cells. Flow cytometry analysis of fixed and permeabilized human PC-3 cells using Glycerol-3-phosphate Phosphatase Antibody at 1 ug per million cells. The antibody-stained population (blue) shows a rightward fluorescence shift relative to the isotype control (green) and unstained cells (red), demonstrating antibody-associated staining consistent with PGP detection. Cells were blocked with goat serum before staining.