PGP9.5 Antibody / Ubiquitin Hydrolase Pathway Antibody [clone UCHL1/775] (V2920)

Description

Protein gene product 9.5 (PGP9.5), also known as Ubiquitin C-terminal hydrolase L1 (UCHL1), is a cytoplasmic deubiquitinating enzyme that plays a central role in the ubiquitin pathway and regulation of intracellular protein turnover. The PGP9.5 Antibody / Ubiquitin Hydrolase Pathway Antibody (clone UCHL1/775) is specifically suited for studies focused on ubiquitin-mediated protein regulation, enabling detection of UCHL1 in systems where deubiquitination and protein degradation pathways are under investigation.

PGP9.5 Antibody, also referred to as UCHL1 antibody or ubiquitin C-terminal hydrolase L1 antibody, is widely used in research examining the ubiquitin-proteasome system and the control of protein stability. UCHL1 functions as a deubiquitinating enzyme that hydrolyzes ubiquitin from protein substrates, maintaining free ubiquitin pools and regulating the fate of proteins targeted for degradation. This positions UCHL1 as a key regulator within the ubiquitin pathway, directly influencing protein turnover and intracellular signaling dynamics.

This mouse monoclonal PGP9.5 Antibody (clone UCHL1/775) supports detection of UCHL1 in experimental models focused on ubiquitin signaling and protein degradation mechanisms. The antibody enables clear visualization of cytoplasmic UCHL1 expression, which reflects active involvement in deubiquitination and ubiquitin recycling processes. Its use supports studies aimed at understanding how disruption of the ubiquitin pathway impacts cellular homeostasis and regulatory protein turnover.

Alterations in deubiquitination and ubiquitin pathway activity are closely linked to disease mechanisms, including neurodegenerative disorders, cancer progression, and cellular stress responses. UCHL1 is therefore frequently studied in the context of ubiquitin-mediated regulation of protein quality control and degradation pathways. Detection of UCHL1 provides insight into how cells regulate protein stability through the balance of ubiquitination and deubiquitination.

A PGP9.5 Antibody / Ubiquitin Hydrolase Pathway Antibody (clone UCHL1/775) is ideally suited for research applications focused on ubiquitin signaling, deubiquitination, and protein turnover. Its positioning as a ubiquitin pathway-focused antibody clearly differentiates it from neuronal marker-oriented reagents and supports mechanistic investigation of protein degradation and intracellular regulatory pathways.

Application Notes

Optimal dilution of the PGP9.5 Antibody / Ubiquitin Hydrolase Pathway Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the PGP9.5 Antibody / Ubiquitin Hydrolase Pathway Antibody.

Storage

Store the PGP 9.5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

UCHL1 antibody, PGP9.5 antibody, Ubiquitin C-terminal hydrolase L1 antibody, Deubiquitinating enzyme UCHL1 antibody