PGLYRP4 Antibody / Peptidoglycan recognition protein 4 Antibody (FY13543)

PGLYRP4 Antibody by WB in human and rodent samples. Western blot analysis using Peptidoglycan recognition protein 4 Antibody detects a prominent band at approximately 45 kDa in the tested lysates. Lanes 1 and 2 contain human HEL and K562 whole-cell lysates; lanes 3 and 4 contain rat testis and stomach; lanes 5 and 6 contain mouse testis and stomach. Each lane received 30 ug reduced protein, resolved by 10% SDS-PAGE and transferred to nitrocellulose. The primary antibody was incubated at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection. The principal signal occurs at a comparable molecular weight across samples.

PGLYRP4 Antibody by FACS in K562 cells. Flow cytometry analysis using Peptidoglycan recognition protein 4 Antibody demonstrates a rightward shift of the blue antibody-stained K562 cell population compared with the green isotype control and red unstained control. Cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum before incubation with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. Fluorescent anti-rabbit secondary antibody was applied for an additional 30 minutes. The histogram illustrates PGLYRP4-associated staining relative to the controls in this fixed-cell assay.