PGAM2 Antibody / Phosphoglycerate mutase 2 (FY13457)

Immunohistochemistry analysis of PGAM2 using PGAM2 antibody. PGAM2 expression was examined in a paraffin-embedded section of human heart tissue, with immunoreactivity predominantly observed in cardiomyocytes, consistent with the high metabolic demand of cardiac muscle. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PGAM2 antibody overnight at 4°C. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Immunofluorescence analysis of PGAM2 using PGAM2 antibody. PGAM2 expression was examined in human HeLa cells, with signal primarily observed in the cytoplasm, consistent with the cytosolic localization of this glycolytic enzyme. Cells were blocked with normal goat serum and incubated with PGAM2 antibody (green) overnight at 4°C. Immunoreactivity was visualized using fluorescence detection, and nuclei were counterstained with DAPI (blue).

Western blot analysis of PGAM2 using PGAM2 antibody. PGAM2 expression was examined in rat heart tissue lysates and mouse heart and testis tissue lysates. Lane 1 shows rat heart tissue lysate, Lane 2 shows mouse heart tissue lysate, and Lane 3 shows mouse testis tissue lysate. PGAM2 was detected as a single band migrating at approximately 29 kDa, consistent with the predicted molecular weight of approximately 29 kDa for Phosphoglycerate mutase 2. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.

Flow cytometry analysis of PGAM2 using PGAM2 antibody. PGAM2 expression was examined in human HeLa cells following fixation with 4% paraformaldehyde and permeabilization to enable intracellular staining. Cells were incubated with PGAM2 antibody and detected using a fluorescent secondary antibody (blue). An isotype control stained under identical conditions is shown in green, and an unstained control is shown in red. The rightward fluorescence shift observed with PGAM2 antibody staining indicates specific intracellular detection of PGAM2.