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Home >> Antibodies >> PFN2 Antibody / Profilin 2

PFN2 Antibody / Profilin 2 (RQ6332)

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  Catalog No Formulation Size Price (USD)  
Image RQ6332 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human renal cancer with PFN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human renal cancer with PFN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with PFN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with PFN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-87 MG, 2) PC-3, 3) A549, 4) HeLa, 5) HEK293, 6) rat brain, 7) mouse brain and 8) mouse kidney lysate with PFN2 antibody. Predicted molecular weight ~15 kDa.
Flow cytometry testing of human A431 cells with PFN2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PFN2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P35080
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PFN2 antibody is available for research use only.
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  • Applications : WB, IHC-P, FACS
    Reactivity : Human, Mouse, Rat

Description

Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Application Notes

Optimal dilution of the PFN2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E96-S138) was used as the immunogen for the PFN2 antibody.

Storage

After reconstitution, the PFN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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