PFKL Antibody / ATP-dependent 6-phosphofructokinase, liver type Antibody (FY13530)

PFKL Antibody by IF in human SiHa cells. Immunofluorescence staining using ATP-dependent 6-phosphofructokinase, liver type Antibody demonstrates green signal in the cytoplasm of SiHa cells, with particularly bright fluorescence around portions of the nuclear periphery and scattered punctate cytoplasmic regions. DAPI counterstaining identifies nuclei in blue and highlights the predominantly extranuclear distribution. Cells underwent enzymatic antigen retrieval and overnight incubation with primary antibody at 5 ug/mL at 4oC, followed by detection with a fluorescent anti-rabbit secondary antibody. The image illustrates PFKL-associated immunoreactivity in cultured cervical carcinoma cells.

PFKL Antibody by WB in rat and mouse brain. Western blot analysis using ATP-dependent 6-phosphofructokinase, liver type Antibody detects a prominent band near 78-80 kDa in rat brain (lane 1) and mouse brain (lane 2), consistent with the expected approximately 85 kDa molecular weight of PFKL. Reduced brain lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE. Primary antibody incubation was performed at 0.5 ug/mL overnight at 4oC, followed by chemiluminescent detection. The observed signal supports detection of PFKL in brain tissue from both species under the stated experimental conditions.