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Home >> Antibodies >> PFAS Antibody / Phosphoribosylformylglycinamidine synthase

PFAS Antibody / Phosphoribosylformylglycinamidine synthase (F54807)

  Catalog No Formulation Size Price (USD)  
Image F54807-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54807-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) 293T and 2) Jurkat cell lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
Western blot testing of human HepG2 cell lysate with PFAS antibody. Predicted molecular weight ~145 kDa.
IHC testing of FFPE human brain tissue with PFAS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with PFAS antibody; Blue=isotype control, Green= PFAS antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15067
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This PFAS antibody is available for research use only.
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Description

Phosphoribosylformylglycinamidine synthase is involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity). [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the PFAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 184-213 from the human protein was used as the immunogen for the PFAS antibody .

Storage

Aliquot the PFAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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