- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
FGAMS Antibody / PFAS Polyclonal Antibody (F54807)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
F54807-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 439 | |
|
|
F54807-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 205 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity purified |
| UniProt | O15067 |
| Localization | Cytoplasmic |
| Applications | Flow cytometry: 1:10-1:50 (1x10e6 cells) Immunohistochemistry (FFPE): 1:50-1:100 Western blot: 1:500-1:1000 |
| Limitations | This FGAMS Antibody / PFAS Polyclonal Antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
FGAMS Antibody / PFAS Polyclonal Antibody recognizes phosphoribosylformylglycinamidine synthase, an enzyme involved in the de novo synthesis of purine nucleotides. Encoded by the PFAS gene, FGAMS catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) into formylglycinamidine ribonucleotide (FGAM). This reaction incorporates nitrogen derived from glutamine into a developing purine ring and represents the fourth enzymatic step in the pathway leading to inosine monophosphate (IMP), the common precursor of adenine and guanine nucleotides.
FGAMS is a glutamine amidotransferase that couples glutamine hydrolysis to the modification of a nucleotide intermediate. Its catalytic activity requires ATP and supports the progressive assembly of the purine ring on a ribose phosphate scaffold. Purine nucleotides are essential for nucleic acid synthesis, cellular energy transfer, and numerous signaling processes. Consequently, regulation of this biosynthetic pathway is closely connected to nutrient availability, cellular proliferation, and metabolic demands. Cells can obtain purines through salvage reactions, but de novo synthesis provides an additional source when nucleotide requirements increase.
An important aspect of FGAMS biology is its participation in purinosomes, transient assemblies containing enzymes of the de novo purine biosynthetic pathway. These structures have been observed under conditions that favor increased purine production, including purine depletion. FGAMS has been used in studies examining purinosome formation, intracellular organization, and the relationship between enzyme localization and metabolic activity. Although purinosomes are associated with coordinated nucleotide synthesis, their precise organization and functional contributions remain subjects of investigation.
FGAMS is primarily associated with the cytoplasm, where de novo purine biosynthesis takes place. Its activity contributes to the nucleotide supply required for DNA replication, RNA synthesis, and other processes dependent on purine-containing molecules. Altered purine metabolism has attracted attention in cancer research because proliferating cells frequently exhibit increased nucleotide requirements. Investigating FGAMS can therefore help clarify how nucleotide biosynthesis responds to metabolic conditions and how purinosome-associated enzymes contribute to cellular homeostasis. NSJ Bioreagents offers this FGAMS Antibody for research into purine metabolism, enzyme organization, and the regulation of intracellular nucleotide production.
For additional research options, explore our PFAS Antibody, which has been evaluated by Western blot across a broad panel of human, mouse, and rat samples.
The stated application concentrations are suggested starting points. Titration of the FGAMS Antibody / PFAS Polyclonal Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 184-213 from the human protein was used as the immunogen for the PFAS / FGAMS antibody.
Aliquot the PFAS / FGAMS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Phosphoribosylformylglycinamidine synthase antibody, FGAR amidotransferase antibody, Formylglycinamidine ribonucleotide synthase antibody, Phosphoribosylformylglycinamidine amidotransferase antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
