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Home >> Antibodies >> PF4 Antibody / Platelet Activation Marker Antibody

PF4 Antibody / Platelet Activation Marker Antibody [clone ACED-16] (RQ5014)

  Catalog No Formulation Size Price (USD)  
Image RQ5014 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 449
Microvalidated Recrabbitmono
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PF4 Antibody Human Spleen WB. Western blot testing of human spleen tissue lysate with PF4 Antibody. A specific band was detected at approximately 10 kDa, consistent with the expected migration range of Platelet Factor 4. PF4 has a predicted monomeric molecular weight of approximately 8 kDa but may also be detected as higher molecular weight dimeric or tetrameric forms due to its natural oligomerization properties. PF4 Antibody / Platelet Activation Marker Antibody supports analysis of platelet-derived chemokine expression, alpha granule biology and platelet activation signaling pathways.
PF4 Antibody Rat Tissue WB. Western blot testing of rat spleen and rat lung tissue lysates with PF4 Antibody. Lane 1: rat spleen; Lane 2: rat lung. A specific band was detected at approximately 15 kDa, consistent with detection of Platelet Factor 4. PF4 has a predicted monomeric molecular weight of approximately 8-11 kDa and is known to form higher molecular weight oligomers, including dimeric and tetrameric structures. PF4 Antibody / Platelet Activation Marker Antibody supports analysis of platelet-derived chemokine expression, alpha granule biology and platelet activation signaling pathways.
PF4 Antibody Mouse Spleen IHC. Immunohistochemistry staining of FFPE mouse spleen tissue with PF4 Antibody at 1:50 dilution. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-PF4 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. PF4 Antibody / Platelet Activation Marker Antibody demonstrates staining consistent with platelet-derived chemokine expression and supports studies of platelet activation, alpha granule biology and immune regulation pathways.
PF4 Antibody Mouse Spleen Germinal Center IHC. Immunohistochemistry staining of FFPE mouse spleen tissue with PF4 Antibody at 1:50 dilution. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-PF4 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. PF4 Antibody / Platelet Activation Marker Antibody demonstrates staining of platelet-associated regions consistent with Platelet Factor 4 expression and supports studies of platelet activation, alpha granule release and immune signaling pathways.
PF4 Antibody Rat Spleen IHC. Immunohistochemistry staining of FFPE rat spleen tissue with PF4 Antibody at 1:50 dilution. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-PF4 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. PF4 Antibody / Platelet Activation Marker Antibody demonstrates staining consistent with Platelet Factor 4 expression in platelet-rich regions and supports studies of platelet activation, alpha granule biology and immune signaling pathways.
PF4 Antibody Rat Spleen Megakaryocyte IHC. Immunohistochemistry staining of FFPE rat spleen tissue with PF4 Antibody at 1:50 dilution. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-PF4 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. PF4 Antibody / Platelet Activation Marker Antibody demonstrates strong staining consistent with Platelet Factor 4 expression in megakaryocytes and platelet-associated regions, supporting studies of platelet activation, alpha granule biology and hematopoietic regulation pathways.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name ACED-16
Purity Affinity purified
UniProt P02776
Applications Western Blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This PF4 Antibody / Platelet Activation Marker Antibody is available for research use only.
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Description

PF4 Antibody / Platelet Activation Marker Antibody detects Platelet Factor 4, also known as CXCL4, a platelet-derived chemokine released from alpha granules following platelet activation. PF4 belongs to the CXC chemokine family and is one of the most abundant proteins secreted by activated platelets. It participates in communication between platelets, immune cells and vascular tissues by regulating inflammatory responses, coagulation-associated signaling and cellular interactions within the vascular environment. Because of its strong association with activated platelets, PF4 is widely studied as a marker of platelet function and platelet-mediated biological processes.

Platelet activation triggers the rapid release of PF4 from intracellular storage granules into the surrounding environment, where it interacts with multiple molecules involved in immune and vascular regulation. PF4 binds strongly to heparin-like glycosaminoglycans and influences pathways related to coagulation, inflammation and cell recruitment. The relationship between platelet activation and immune signaling has made PF4 an important target for studies examining the connection between hemostasis and inflammatory responses. PF4 Antibody is commonly used by researchers studying platelet biology, megakaryocyte differentiation, chemokine activity and vascular signaling pathways.

Research involving PF4 has expanded beyond traditional platelet function into areas including immunology, cancer biology and inflammatory disease mechanisms. CXCL4 can influence immune cell behavior, including macrophage activity, cytokine responses and leukocyte interactions. Platelet-derived chemokines such as PF4 contribute to the complex communication network between circulating blood cells and tissue environments. Studies examining PF4 expression help define how platelet activation contributes to immune regulation, vascular remodeling and cellular responses during physiological and disease-associated processes.

PF4 is also frequently investigated as a platelet lineage marker due to its enrichment in megakaryocytes and platelet alpha granules. Analysis of PF4 expression supports research into hematopoietic differentiation, platelet production and molecular events associated with platelet activation states. As platelets are increasingly recognized as regulators of inflammation and immune communication, PF4 remains an important molecule for understanding interactions between coagulation pathways and immune system activity.

PF4 Antibody applications include detection of Platelet Factor 4 expression, localization and regulation in biological samples using techniques such as Western blot, immunohistochemistry, immunofluorescence and related protein analysis methods. Evaluation of PF4 supports studies of platelet activation, alpha granule biology, chemokine signaling, inflammation and vascular biology. NSJ Bioreagents provides PF4 Antibody / Platelet Activation Marker Antibody, clone ACED-16, for researchers investigating platelet function, immune regulation and hematology-related signaling pathways.

PF4 Antibody detects a platelet activation marker involved in alpha granule release, chemokine signaling and immune regulation, making it a useful marker for research areas featured in our Immunology Antibodies page.

Application Notes

Optimal dilution of the PF4 Antibody / Platelet Activation Marker Antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Platelet Factor 4 was used as the immunogen for the PF4 antibody.

Storage

Store the PF4 antibody at -20oC.

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