PEX16 Antibody / Peroxisomal biogenesis factor 16 (FY13456)

Immunofluorescence analysis of Peroxisomal biogenesis factor 16 using PEX16 antibody. PEX16 expression was examined in cultured human HeLa cells. Enzymatic antigen retrieval was performed prior to staining. Cells were blocked with normal goat serum and incubated with PEX16 antibody (green) overnight at 4C. Immunoreactivity shows a cytoplasmic staining pattern with punctate distribution consistent with peroxisomal membranes, and nuclei were counterstained with DAPI (blue).

Western blot analysis of PEX16 using PEX16 antibody. Protein lysates from human A549 cells (Lane 1), human MCF-7 cells (Lane 2), human HeLa cells (Lane 3), human HepG2 cells (Lane 4), rat RH35 cells (Lane 5) and mouse liver tissue (Lane 6) were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. PEX16 was detected as a band migrating at approximately 42 kDa, slightly above the predicted molecular weight of approximately 39 kDa for Peroxisomal biogenesis factor 16, consistent with modest anomalous migration commonly observed for peroxisomal membrane-associated proteins. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.

Immunoprecipitation and western blot analysis of PEX16 using PEX16 antibody. PEX16 was immunoprecipitated from human MCF-7 whole cell lysates and analyzed by SDS-PAGE followed by immunoblotting. Lane 1 shows human MCF-7 whole cell lysate input, Lane 2 shows immunoprecipitation performed using rabbit control IgG, and Lane 3 shows immunoprecipitation performed using PEX16 antibody. PEX16 was detected as a band migrating at approximately 42 kDa, slightly above the predicted molecular weight of approximately 39 kDa for Peroxisomal biogenesis factor 16. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.