PERK Antibody / Endoplasmic Reticulum Stress Kinase Antibody (RQ4206)

PERK Antibody Human Pancreatic Cancer IHC. Immunohistochemistry was performed on FFPE human pancreatic cancer tissue using PERK Antibody. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to incubation with the primary antibody at 2 ug/ml. Bound antibody was visualized using an HRP-conjugated goat anti-rabbit IgG secondary antibody with DAB chromogen. Moderate cytoplasmic staining is observed in the malignant glandular epithelial cells, with comparatively weaker staining in the surrounding stromal tissue, consistent with the elevated endoplasmic reticulum stress response frequently observed in pancreatic cancer. These results demonstrate that this Endoplasmic Reticulum Stress Kinase Antibody is suitable for immunohistochemical detection of PERK (EIF2AK3) in formalin-fixed, paraffin-embedded human pancreatic cancer tissue.

PERK Antibody Human Cancer Cell WB. Western blot analysis was performed using PERK Antibody. Lane 1: human K562 whole cell lysate; lane 2: human 293T whole cell lysate; lane 3: human MCF-7 whole cell lysate; lane 4: human HepG2 whole cell lysate. Proteins were separated on an 8% SDS-PAGE gel, transferred to a nitrocellulose membrane, and incubated with the primary antibody at 0.5 ug/ml overnight at 4°C. A specific immunoreactive band is detected at approximately 150 kDa in all tested cell lines, consistent with the commonly observed migration of glycosylated PERK, although the predicted molecular weight is approximately 125 kDa. These results demonstrate that this Endoplasmic Reticulum Stress Kinase Antibody is suitable for Western blot detection of PERK (EIF2AK3) in human cell lysates.