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Home >> Antibodies >> Periostin Antibody

Periostin Antibody (R32897)

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Image R32897 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat stomach, 2) rat lung, 3) rat NRK cells, 4) mouse heart, 5) mouse intestine and 6) mouse lung lysate with Periostin antibody at 0.5ug/ml. Predicted molecular weight ~93 kDa.
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Western blot testing of 1) rat stomach, 2) rat lung, 3) rat NRK cells, 4) mouse heart, 5) mouse intestine and 6) mouse lung lysate with Periostin antibody at 0.5ug/ml. Predicted molecular weight ~93 kDa.
IHC testing of FFPE mouse stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat stomach tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lung tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with Periostin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt D3ZAF5
Localization Cytoplasm, extracellular space
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA (recombinant rat protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This Periostin antibody is available for research use only.
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Description

POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Application Notes

Optimal dilution of the Periostin antibody should be determined by the researcher.

Immunogen

A recombinant rat protein corresponding to amino acids K577-Q810 was used as the immunogen for the Periostin antibody.

Storage

After reconstitution, the Periostin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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