Periostin Antibody for IHC / POSTN [clone POSTN/3501] (V8696)

Immunohistochemistry of Periostin antibody for IHC in human placenta. Formalin-fixed, paraffin-embedded human placenta was stained with mouse monoclonal Periostin antibody (clone POSTN/3501) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal is observed predominantly in stromal and extracellular matrix regions within chorionic villi, consistent with the secreted and matrix-associated localization of Periostin, while trophoblastic nuclei remain largely negative.

Immunohistochemistry of Periostin antibody for IHC in human colon carcinoma. Formalin-fixed, paraffin-embedded human colon carcinoma tissue was stained with mouse monoclonal Periostin antibody (clone POSTN/3501) following heat-induced epitope retrieval by boiling in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes and cooling prior to testing. HRP-DAB brown chromogenic signal highlights abundant stromal and extracellular matrix regions surrounding malignant glands, consistent with the secreted and matrix-associated localization of Periostin, while tumor cell nuclei remain largely negative.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Periostin antibody (clone POSTN/3501). These results demonstrate the foremost specificity of the POSTN/3501 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Periostin antibody (clone POSTN/3501) as confirmation of integrity and purity.