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Home >> Antibodies >> PER1 Antibody

PER1 Antibody (RQ4051)

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  Catalog No Formulation Size Price (USD)  
Image RQ4051 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human PANC-1, 2) human MCF7, 3) human COLO320, 4) rat heart and 5) mouse brain lysate with PER1 antibody at 0.5ug/ml. Predicted molecular weight ~136 kDa, observed here at ~200 kDa.
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Western blot testing of 1) human PANC-1, 2) human MCF7, 3) human COLO320, 4) rat heart and 5) mouse brain lysate with PER1 antibody at 0.5ug/ml. Predicted molecular weight ~136 kDa, observed here at ~200 kDa.
IHC testing of FFPE rat brain tissue with PER1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O15534
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This PER1 antibody is available for research use only.
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Description

The PER1 gene encodes the period circadian protein homolog 1 protein in humans. This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described.

Application Notes

Optimal dilution of the PER1 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids D1119-M1200 was used as the immunogen for the PER1 antibody.

Storage

After reconstitution, the PER1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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