PEPCK2 Antibody / PEPCK-M Antibody [clone 3F7] (RQ6225)

PEPCK2 Antibody HepG2 Liver Cancer Cell IF. PEPCK2 was detected in formalin-fixed, paraffin-embedded human HepG2 liver cancer cells following heat-induced epitope retrieval by steaming in citrate buffer, pH 6.0, for 20 minutes. Immunofluorescent staining with PEPCK2 antibody clone 3F7 shows the target protein in green, while nuclei were counterstained with DAPI and appear blue. The predominantly granular cytoplasmic staining pattern is consistent with the mitochondrial localization of PEPCK2. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports studies of mitochondrial carbon metabolism and metabolic adaptation in liver cancer cells.

PEPCK2 Antibody Human Prostate Cancer IHC. PEPCK2 was detected in a formalin-fixed, paraffin-embedded section of human prostate cancer tissue. Heat-induced epitope retrieval was performed by boiling the section in EDTA buffer, pH 8.0, for 20 minutes followed by cooling before staining with PEPCK2 antibody clone 3F7. Brown chromogenic signal is primarily localized to the cytoplasm of malignant glandular epithelial cells, with limited staining in the surrounding stromal tissue. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports studies of mitochondrial carbon metabolism and metabolic adaptation in prostate cancer.

PEPCK2 Antibody Human Breast Cancer IHC. PEPCK2 was detected in a formalin-fixed, paraffin-embedded section of human breast cancer tissue. Heat-induced epitope retrieval was performed by boiling the section in EDTA buffer, pH 8.0, for 20 minutes followed by cooling before staining with PEPCK2 antibody clone 3F7. Brown chromogenic signal is visible in the cytoplasm of malignant breast epithelial cells, with variable staining intensity across the tumor and limited stromal reactivity. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports studies of mitochondrial metabolism and nutrient adaptation in breast cancer.

PEPCK2 Antibody Human Rectal Cancer IHC. PEPCK2 was detected in a formalin-fixed, paraffin-embedded section of human rectal cancer tissue. Heat-induced epitope retrieval was performed by boiling the section in EDTA buffer, pH 8.0, for 20 minutes followed by cooling before staining with PEPCK2 antibody clone 3F7. Weak, variable brown chromogenic signal is visible in the cytoplasm of malignant glandular epithelial cells, while nuclei are counterstained blue. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports studies of mitochondrial enzyme expression and metabolic adaptation in colorectal cancer.

PEPCK2 Antibody Human, Monkey, Rat and Mouse WB. Western blot analysis was performed using lysates from human HepG2 cells in lane 1, human HeLa cells in lane 2, human HEK293 cells in lane 3, human A431 cells in lane 4, monkey COS-7 cells in lane 5, rat kidney in lane 6, mouse kidney in lane 7 and mouse Neuro-2a cells in lane 8. PEPCK2 antibody clone 3F7 detected a band near the predicted molecular weight of 71 kDa across the tested samples, with an additional lower band in mouse kidney. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports multi-species analysis of mitochondrial PEPCK expression.

PEPCK2 Antibody MCF7 Breast Cancer Cell FACS. Flow cytometry analysis was performed using human MCF7 breast cancer cells blocked with goat serum and stained with PEPCK2 antibody clone 3F7 at 1 ug per million cells. The red trace represents cells alone, the green trace represents the isotype control and the blue trace represents PEPCK2 antibody staining. The blue population shows a modest rightward shift relative to the isotype control, demonstrating detectable PEPCK2 expression in MCF7 cells. This PEPCK2 Antibody, also described as a PEPCK-M Antibody, supports metabolic protein analysis by flow cytometry.