PEMT Antibody / Phosphatidylethanolamine N-methyltransferase Antibody (FY13518)

PEMT Antibody by IHC in mouse stomach tissue. Immunohistochemistry of paraffin-embedded mouse stomach using Phosphatidylethanolamine N-methyltransferase Antibody demonstrates prominent granular brown staining in groups of epithelial cells, with blue hematoxylin-counterstained nuclei. Signal intensity varies across the tissue, with some regions showing little staining. Sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, followed by blocking with goat serum and incubation with 2 ug/mL primary antibody overnight at 4oC. An HRP-conjugated anti-rabbit detection system with DAB chromogen was used. The image illustrates PEMT-associated immunoreactivity within gastric tissue.

PEMT Antibody by WB in rat and mouse liver. Western blot analysis using Phosphatidylethanolamine N-methyltransferase Antibody detects a band near 18 kDa in rat liver (lane 1) and mouse liver (lane 2). This migration agrees with published purification studies reporting an approximately 18.3 kDa PEMT protein, although the canonical sequence predicts a mass closer to 22 kDa. An additional prominent band near 30 kDa is uncharacterized; its identity cannot be assigned from the available data. Each lane contained 30 ug of reduced lysate separated on a 12% SDS-PAGE gel. Primary antibody was applied at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

PEMT Antibody by IHC in human prostate carcinoma. Immunohistochemistry of paraffin-embedded human prostate carcinoma using Phosphatidylethanolamine N-methyltransferase Antibody shows brown staining in epithelial structures, with variable signal intensity across glands and blue hematoxylin-counterstained nuclei. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, and blocking with goat serum. The primary antibody was applied at 2 ug/mL overnight at 4oC, followed by an HRP-linked anti-rabbit detection system and DAB chromogen. The image demonstrates a heterogeneous distribution of PEMT-associated immunoreactivity within the examined tumor tissue.

PEMT Antibody by FACS in MCF-7 cells. Flow cytometry of fixed and permeabilized MCF-7 cells using Phosphatidylethanolamine N-methyltransferase Antibody shows a rightward fluorescence shift of the blue antibody-stained population relative to the green isotype control and red unstained blank. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with normal goat serum. The primary antibody was applied at 1 ug per 1 x 10^6 cells, followed by fluorescent anti-rabbit secondary antibody detection. The histogram supports intracellular PEMT-associated staining in this cell line.