PELO Antibody / Protein pelota homolog (FY13452)

Immunohistochemistry analysis of Protein pelota homolog using PELO antibody. PELO expression was examined in a paraffin-embedded section of rat kidney tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PELO antibody overnight at 4C. Immunoreactivity is observed predominantly in renal tubular epithelial cells, with minimal staining in surrounding stromal areas. Detection was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Immunohistochemistry analysis of Protein pelota homolog using PELO antibody. PELO expression was examined in a paraffin-embedded section of human breast cancer tissue. Heat-mediated antigen retrieval was performed using EDTA buffer (pH 8.0). Tissue sections were blocked with normal goat serum and incubated with PELO antibody overnight at 4C. Immunoreactivity is observed within tumor epithelial cells, consistent with cytoplasmic localization, with variable staining intensity across the tumor field. Detection was visualized using an HRP-based detection system with DAB chromogen, followed by hematoxylin counterstaining.

Western blot analysis of PELO using PELO antibody. Protein lysates from human U251 cells (Lane 1), human HeLa cells (Lane 2), human A549 cells (Lane 3), human PC-3 cells (Lane 4), rat lung tissue (Lane 5), rat kidney tissue (Lane 6), mouse lung tissue (Lane 7), and mouse kidney tissue (Lane 8) were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. PELO was detected as a band at approximately 43 kDa, consistent with the predicted molecular weight of Protein pelota homolog. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.