PELI1 Antibody / E3 ubiquitin-protein ligase pellino homolog 1 Antibody (FY13528)

PELI1 Antibody by IHC in rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded rat lung using E3 ubiquitin-protein ligase pellino homolog 1 Antibody shows conspicuous brown staining along the epithelial lining of a large airway. Immunoreactivity is predominantly cytoplasmic and apical, while hematoxylin counterstaining reveals the blue nuclei of airway epithelial and surrounding cells. Antigen retrieval was performed in EDTA buffer, pH 8.0, followed by overnight primary antibody incubation at 2 ug/mL at 4oC and HRP/DAB detection. The strong epithelial signal and clearly defined airway lumen make this image useful for evaluating PELI1-associated staining in lung tissue.

PELI1 Antibody by WB across human and rodent samples. Western blot analysis using E3 ubiquitin-protein ligase pellino homolog 1 Antibody detects a principal band near 40 kDa in human THP-1 (lane 1) and Caco-2 (lane 2) cells, rat thymus (lane 3) and lung (lane 4), and mouse thymus (lane 5) and lung (lane 6). The observed migration is modestly below the approximately 46 kDa predicted molecular weight. Signal is particularly strong in THP-1 and lung samples and weaker in rat thymus. Each lane contained 30 ug of reduced lysate separated by 10% SDS-PAGE. Primary antibody was applied at 0.5 ug/mL overnight at 4oC before chemiluminescent visualization.

PELI1 Antibody by IP from human Caco-2 cells. Immunoprecipitation of Caco-2 lysate using E3 ubiquitin-protein ligase pellino homolog 1 Antibody enriches a band near 43 kDa in the antibody immunoprecipitate (lane 3) compared with the control IgG immunoprecipitate (lane 2). The input lysate (lane 1) also contains a band in the corresponding region. Lane 1 contains 30 ug of input protein, while the immunoprecipitation used 500 ug of lysate and 2 ug of antibody. Additional bands are present in the input and immunoprecipitated fractions. The comparison supports enrichment of PELI1-associated material relative to the IgG control.

PELI1 Antibody by IHC in mouse lung. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse lung using E3 ubiquitin-protein ligase pellino homolog 1 Antibody reveals brown staining along the epithelium lining a pulmonary airway, with additional scattered staining in surrounding tissue. The epithelial signal is most conspicuous near the luminal surface, while hematoxylin counterstaining highlights blue nuclei. Sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, and overnight primary antibody incubation at 2 ug/mL at 4oC, followed by HRP/DAB detection. This image complements the rat lung result by showing PELI1-associated immunoreactivity in mouse respiratory epithelium.

PELI1 Antibody by IHC in human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded human skin using E3 ubiquitin-protein ligase pellino homolog 1 Antibody demonstrates brown staining within the epidermal layers, with stronger reaction product in portions of the differentiated epithelial compartment. Hematoxylin counterstaining identifies nuclei along the basal and suprabasal regions and provides contrast with the pale dermal tissue. Antigen retrieval was carried out in EDTA buffer, pH 8.0, followed by primary antibody incubation at 2 ug/mL overnight at 4oC and HRP/DAB visualization. The tissue section illustrates PELI1-associated immunoreactivity within stratified squamous epithelium.

PELI1 Antibody by FACS in human THP-1 cells. Intracellular flow cytometry using E3 ubiquitin-protein ligase pellino homolog 1 Antibody shows a rightward shift of the blue antibody-stained histogram relative to the green isotype-control and red blank-control populations in THP-1 cells. Cells were fixed with paraformaldehyde, permeabilized, blocked with goat serum, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. A fluorescent anti-rabbit secondary antibody enabled detection. The clearly separated distributions indicate PELI1-associated staining above the control signals.

PELI1 Antibody by FACS in rat PC-12 cells. Intracellular flow cytometry using E3 ubiquitin-protein ligase pellino homolog 1 Antibody reveals a blue fluorescence peak shifted rightward relative to the green isotype-control and red blank-control peaks in PC-12 cells. Following fixation and permeabilization, cells were blocked with goat serum and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by a fluorescent anti-rabbit secondary antibody. The distribution supports detectable PELI1-associated intracellular staining in the examined rat cell population.