PECAM1 Antibody PECAM1/3534 / CD31 Monoclonal Antibody [clone PECAM1/3534] (V8845)

PECAM1 Antibody PECAM1/3534 in Human Tonsil Vasculature by IHC. Immunohistochemistry of FFPE human tonsil sections using PECAM1 Antibody clone PECAM1/3534. Distinct brown immunoreactivity highlights numerous endothelial-lined vascular structures distributed through the tissue, while surrounding lymphoid regions show substantially less staining. The pattern provides clear visualization of the characteristic vascular distribution of CD31. HIER was performed by boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allowing them to cool before testing. The selective endothelial staining supports tissue detection with this CD31 Monoclonal Antibody.

PECAM1 Antibody PECAM1/3534 in Human Colon Vasculature by IHC. Immunohistochemistry of FFPE human colon sections using PECAM1 Antibody clone PECAM1/3534. Strong brown staining delineates an extensive network of endothelial-lined vascular structures, producing pronounced contrast with adjacent tissue. The distribution is consistent with localization of the vascular adhesion molecule CD31/PECAM-1. HIER was performed by boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allowing them to cool before testing. This result provides additional tissue-based validation of the CD31 Monoclonal Antibody.

PECAM1 Antibody PECAM1/3534 in Jurkat Cells by FACS. Flow cytometry analysis of human Jurkat cells using PECAM1 Antibody clone PECAM1/3534. Antibody staining is shown in blue and the isotype control in red. The CD31-stained population displays a pronounced rightward fluorescence shift relative to the control, with clear separation between the distributions. The result demonstrates detection of cell-associated PECAM-1 and provides flow cytometry validation complementary to the tissue staining, western blot, immunofluorescence and protein microarray data available for this CD31 Monoclonal Antibody.

PECAM1 Antibody PECAM1/3534 in THP-1 Cells by WB. Western blot analysis of human THP-1 cell lysate using PECAM1 Antibody clone PECAM1/3534. A strong, discrete immunoreactive band is visible in the approximately 100-130 kDa region. CD31 is extensively glycosylated and has an expected apparent molecular weight of approximately 83-130 kDa depending on glycosylation state, placing the observed signal within the expected range for endogenous PECAM-1. The clean band provides biochemical validation of this CD31 Monoclonal Antibody and complements its cell- and tissue-based validation data.

PECAM1 Antibody PECAM1/3534 in Jurkat Cells by IF. Immunofluorescence analysis of human Jurkat cells using PECAM1 Antibody clone PECAM1/3534. Green CD31 immunoreactivity prominently outlines the cellular periphery, consistent with localization of PECAM-1 at the plasma membrane. RedDot nuclear staining is shown in red and provides clear contrast with the green antibody signal. The well-defined peripheral staining across numerous cells demonstrates cellular localization of PECAM-1 and provides fluorescence-based validation of this CD31 Monoclonal Antibody.

PECAM1 Antibody PECAM1/3534 Protein Microarray Validation. Protein microarray analysis of PECAM1 Antibody clone PECAM1/3534 using a HuProt array containing more than 19,000 full-length human proteins. PECAM1 produces the dominant array signal, visibly separated from the next-highest protein signals. The Z-score represents target signal strength in standard deviations above the mean array signal, while the S-score measures separation between the target Z-score and the next-highest signal. The strong preferential recognition of PECAM1 supports designation of this antibody as Protein Microarray Validated and provides orthogonal specificity evidence for the CD31 Monoclonal Antibody.