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Home >> Antibodies >> PECAM-1 Antibody / CD31

PECAM-1 Antibody / CD31 [clone 158-2B3] (V2783)

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  Catalog No Formulation Size Price (USD)  
Image V2783-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2783-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2783SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human Jurkat cells with PECAM-1 antibody (green, clone 158-2B3) and Reddot nuclear counterstain (red).
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Immunofluorescent staining of PFA-fixed human Jurkat cells with PECAM-1 antibody (green, clone 158-2B3) and Reddot nuclear counterstain (red).
Flow cytometry testing of PFA-fixed human Jurkat cells with PECAM-1 antibody (clone 158-2B3); Red=isotype control, Blue= PECAM-1 antibody.
SDS-PAGE analysis of purified, BSA-free PECAM-1 antibody (clone 158-2B3) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 158-2B3
Purity Protein G affinity chromatography
UniProt P16284
Localization Cell surface and cytoplasm of endothelial cells
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Limitations This PECAM-1 antibody is available for research use only.
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Description

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Application Notes

Optimal dilution of the PECAM-1 antibody should be determined by the researcher.

Immunogen

Stimulated human leukocytes (Workshop VI) were used as the immunogen for the PECAM-1 antibody.

Storage

Store the PECAM-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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