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Home >> Antibodies >> PEA3 Antibody / ETV4

PEA3 Antibody / ETV4 (F54869)

  Catalog No Formulation Size Price (USD)  
Image F54869-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54869-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PEA3 antibody.
Western bloot testing of mouse heart tissue lysate with PEA3 antibody. Expected molecular weight: 54-61 kDa.
IHC testing of FFPE human brain tissue with PEA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human WiDr cells with PEA3 antibody; Blue=isotype control, Green= PEA3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P43268
Localization Nuclear
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This PEA3 antibody is available for research use only.
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  • Applications : WB
    Reactivity : Human, Rat

Description

ETV4/PEA3 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PEA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 419-447 from the human protein was used as the immunogen for the PEA3 antibody.

Storage

Aliquot the PEA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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