Pancreatic and Duodenal Homeobox 1 Antibody / IPF1 Antibody (FY13210)

Pancreatic and Duodenal Homeobox 1 Antibody Detection in Human HeLa Cells by IF. Immunofluorescence staining of human HeLa cells using Pancreatic and Duodenal Homeobox 1 Antibody shows red PDX1-associated fluorescence concentrated predominantly within the nuclear region, while beta tubulin is visualized in green throughout the cytoplasmic microtubule network. Cells underwent enzyme antigen retrieval for 15 min before blocking with 10% goat serum and incubation with 5 ug/ml rabbit anti-PDX1 antibody overnight at 4oC. The predominantly nuclear PDX1 signal is consistent with its function as a transcription factor and demonstrates cellular detection using this IPF1 Antibody.

Pancreatic and Duodenal Homeobox 1 Antibody Detection in PC-3, HeLa and 293T Cells by WB. Western blot testing with Pancreatic and Duodenal Homeobox 1 Antibody shows a single prominent band at approximately 40 kDa in human PC-3 (lane 1), HeLa (lane 2), and 293T (lane 3) cell lysates. Although the calculated molecular weight of PDX1 is approximately 31 kDa, migration in the 40-46 kDa range has been associated with post-translational modifications including phosphorylation and SUMOylation. The clean, consistent bands across all three lysates demonstrate detection of endogenous PDX1 using this IPF1 Antibody.

Pancreatic and Duodenal Homeobox 1 Antibody Detection in Human Caco-2 Colorectal Adenocarcinoma Cells by FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 colorectal adenocarcinoma cells using Pancreatic and Duodenal Homeobox 1 Antibody shows a clear rightward fluorescence shift in the PDX1-stained population (blue) relative to the isotype control (green) and unlabeled cells (red). Cells were incubated with antibody at 1 ug/million cells for 30 min at 20oC following fixation, permeabilization, and blocking with normal goat serum. The distinct population shift demonstrates intracellular PDX1 detection using this IPF1 Antibody.